BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//wp-events-plugin.com//6.4.7.2//EN
TZID:Europe/Paris
X-WR-TIMEZONE:Europe/Paris
BEGIN:VEVENT
UID:1-137@lptms.universite-paris-saclay.fr
DTSTART:20130607T140000Z
DTEND:20130607T140000Z
DTSTAMP:20130418T123213Z
URL:http://www.lptms.universite-paris-saclay.fr/seminars/physics-biology-i
 nterface-seminar-david-dulin/
SUMMARY:Physics-Biology interface seminar : David Dulin - Amphi BLANDIN du 
 LPS de la Faculté des Sciences d’Orsay (Bâtiment 510) - 7 Juin 13 14:0
 0
DESCRIPTION:Dynamics of transcription and error incorporation in a viral RN
 A-dependent RNA polymerase\nDavid Dulin (Delft University of Technology)\n
 RNA-dependent RNA polymerases (RdRPs) are essential enzymes that govern tr
 anscription and replication in RNA viruses. While RNA elongation forms an 
 important therapeutic target against viral infection\, little is known abo
 ut elongation dynamics at the single-molecule level. Here\, we study the w
 ell-established RdRP model system of P2 from the double-stranded RNA bacte
 riophage Φ6 using high-throughput single-molecule force-spectroscopy comb
 ined with theoretical modeling. We show that P2 elongation dynamics is irr
 egular\, with rapid transcription repeatedly interrupted by pauses whose d
 urations vary from seconds to thousands of seconds. Exploiting the discrim
 inatory power offered by our large datasets\, together with specifically-a
 dapted analysis\, we introduce a stochastic dynamical model of P2 transcri
 ptional elongation. Our results imply that the majority of pauses result f
 rom nucleotide misincorporation\, providing a direct link between RdRP dyn
 amics and error rates—rates that offer potential drug targets\, as they 
 must be finely tuned to confer both genome stability between generations a
 nd adaptability to bypass host defense systems.
LOCATION:Amphi BLANDIN du LPS de la Faculté des Sciences d’Orsay (Bâtim
 ent 510)\, 15 Rue Georges Clemenceau\, orsay\, France
GEO:48.698187;2.181768
X-APPLE-STRUCTURED-LOCATION;VALUE=URI;X-ADDRESS=15 Rue Georges Clemenceau\,
  orsay\, France;X-APPLE-RADIUS=100;X-TITLE=Amphi BLANDIN du LPS de la Facu
 lté des Sciences d’Orsay (Bâtiment 510):geo:48.698187,2.181768
END:VEVENT
END:VCALENDAR