BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//wp-events-plugin.com//6.4.7.2//EN
TZID:Europe/Paris
X-WR-TIMEZONE:Europe/Paris
BEGIN:VEVENT
UID:1-418@lptms.universite-paris-saclay.fr
DTSTART:20160530T110000Z
DTEND:20160530T120000Z
DTSTAMP:20160414T184706Z
URL:http://www.lptms.universite-paris-saclay.fr/seminars/physics-biology-i
 nterface-seminar-david-pastre/
SUMMARY:Physics-Biology interface seminar: David Pastré - Moyen Amphi\, Bu
 ilding 510\, Université Paris-Saclay Orsay - 30 Mai 16 11:00
DESCRIPTION:Probing protein interactions on a microtubule bench by fluoresc
 ence microscopy: Application to YB-1\, a mRNA-binding protein\nDavid Pastr
 é (Université d'Évry)\nA typical procedure adopted by biologists to ana
 lyze protein interactions is to take advantage of the high throughput capa
 bility of the two hybrid system\, generally in yeast\, or that of the comb
 ination of affinity purification with mass spectrometry to obtain an exhau
 stive list of potential partners for a protein of interest.  However\, the
 se assays require cell lysis\, antibodies and adsorption onto non physiolo
 gical substrates leading to false positives and false negatives. There is 
 therefore a need to control the relevance of the proposed interactions in 
 a context closer to native conditions\, such as in living mammalian cells.
  To that end\, novel methods are currently developed to provide a better v
 iew on protein interactions. In line with this\, we have proposed a novel 
 technology to detect and quantify direct or indirect protein interactions 
 by fluorescence microscopy in living mammalian cells using microtubules as
  platforms. Microtubules are micrometer-long  rigid cylinders of about 25
  nm in diameter that are present in the cytoplasm of all eukaryotic cell
 s. Due to their geometry\, they provide an ideal surface to probe molecula
 r interactions by fluorescence microscopy.  As a proof of concept\, we use
 d microtubules to probe the interactions between mRNA-binding proteins lik
 e YB-1 in the cytoplasm.
CATEGORIES:physbio,seminars
LOCATION:Moyen Amphi\, Building 510\, Université Paris-Saclay Orsay\, 15 R
 ue Georges Clemenceau\, orsay\, France
GEO:48.698187;2.181768
X-APPLE-STRUCTURED-LOCATION;VALUE=URI;X-ADDRESS=15 Rue Georges Clemenceau\,
  orsay\, France;X-APPLE-RADIUS=100;X-TITLE=Moyen Amphi\, Building 510\, Un
 iversité Paris-Saclay Orsay:geo:48.698187,2.181768
END:VEVENT
END:VCALENDAR