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TZID:Europe/Paris
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UID:0-800@lptms.universite-paris-saclay.fr
DTSTART:20211124T110000Z
DTEND:20211124T120000Z
DTSTAMP:20211117T140337Z
URL:http://www.lptms.universite-paris-saclay.fr/seminars/physics-biology-i
 nterface-seminar-diane-laure-pages/
SUMMARY:Physics-Biology interface seminar: Diane-Laure Pagès - Salle des s
 éminaires du FAST et du LPTMS\, bâtiment Pascal n°530 - 24 Nov 21 11:00
DESCRIPTION:Collective amoeboid migration of cancer cell clusters by polari
 sed jiggling\nDiane-Laure Pagès (Institut Gustave Roussy)\nWinner of the 
 PhysBio2021 best short talk award\n\n\nMigration is a key step in many bio
 logical processes\, including the metastatic progression of cancers which 
 accounts for most patient’s deaths. As far as we know\, cell locomotion 
 occurs through three distinct mechanisms. In a few words\, single cells ca
 n migrate via two modes\, mesenchymal (adhesive\, traction-based) or amoeb
 oid (non-adhesive\, propulsion-based). Cell cohorts are generally led by p
 rotrusive leaders\, towing the collective through adhesion to the substrat
 e.\n\nWe have been able to demonstrate the existence of an undescribed mod
 e of collective migration. We study tumour cell clusters’ migration\, tr
 ansformed and non-transformed\, in non-adherent microfabricated channels. 
 This collective migration is independent of focal-adhesions and traction b
 ut is dependent on integrin-mediated friction to the substrate. Moreover\,
  cell clusters display an actomyosin cortex that is polarised to the rear 
 of clusters\, proportionally to migration speed. Inhibiting ROCK and myosi
 n activity decreases migration\, while optogenetic activation of RhoA dict
 ates directionality\, demonstrating that this migration relies on actomyos
 in contractility [2]. However\, such migration is not driven by a sustaine
 d cell or myosin flow. Instead\, we observed fluctuating cell and myosin d
 isplacements that are correlated with clusters’ speed. We then demonstra
 te analytically that\, together with friction with the substrate and myosi
 n polarisation\, this behaviour leads to migration. Our results suggest th
 at cell clusters can use a unique mode of collective migration\, based on 
 “polarised jiggling”\, that may explain the metastatic potential of th
 ese tumour intermediates. We call this new mode of migration “collective
  amoeboid migration”\, by analogy with single cell amoeboid migration.\n
 \n1. Zajac\, O. et al. Tumour spheres with inverted polarity drive the for
 mation of peritoneal metastases in patients with hypermethylated colorecta
 l carcinomas. Nat. Cell Biol. 1 (2018). doi:10.1038/s41556-017-0027-6\n\n2
 . Pagès\, D.-L. et al. Cell clusters adopt a collective amoeboid mode of 
 migration in confined non-adhesive environments. bioRxiv 2020.05.28.106203
  (2020) doi:10.1101/2020.05.28.106203.\n
CATEGORIES:physbio,seminars
LOCATION:Salle des séminaires du FAST et du LPTMS\, bâtiment Pascal n°53
 0\, rue André Riviere\, Orsay\, 91405\, France
X-APPLE-STRUCTURED-LOCATION;VALUE=URI;X-ADDRESS=rue André Riviere\, Orsay\
 , 91405\, France;X-APPLE-RADIUS=100;X-TITLE=Salle des séminaires du FAST 
 et du LPTMS\, bâtiment Pascal n°530:geo:0,0
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