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TZID:Europe/Paris
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BEGIN:VEVENT
UID:1-540@lptms.universite-paris-saclay.fr
DTSTART:20171115T110000Z
DTEND:20171115T120000Z
DTSTAMP:20171030T161108Z
URL:http://www.lptms.universite-paris-saclay.fr/seminars/physics-biology-i
 nterface-seminar-felix-rico/
SUMMARY:Physics-Biology interface seminar: Felix Rico - Amphi BLANDIN du LP
 S de la Faculté des Sciences d’Orsay (Bâtiment 510) - 15 Nov 17 11:00
DESCRIPTION:Molecular to cellular mechanics probed by high-speed atomic for
 ce microscopy\nFelix Rico (Aix-Marseille Université)\n\nThe mechanical pr
 operties of individual proteins\, filaments\, and supramolecular assemblie
 s provide structural stability and mechanical flexibility to the living ce
 ll. Thus\, molecular understanding of the mechanics from the single molecu
 le to the whole cell is relevant to understand biological function. High-s
 peed atomic force microscopy (HS-AFM) is a unique technology that combines
  nanometric-imaging capabilities at video rate. In this talk\, I will pres
 ent our recent applications of HS-AFM to probe protein and cellular mechan
 ics. In the first part\, I will introduce the development of high-speed fo
 rce spectroscopy (HS-FS) to probe protein unfolding at the timescales of m
 olecular dynamics simulations (1). This provides a unique approach to acqu
 ire atomistic understanding of biomolecular processes based on experimenta
 l results. In the second part\, I will present our recent work on the adap
 tation of HS-AFM to probe the microrheology of living cells at high freque
 ncies (up to 100 kHz)\, revealing cytoskeletal dynamics (2). We show that 
 the mechanical response at high frequencies depends on the actin filament 
 tension and pathological state of the cell. Microrheology over a wide dyna
 mic range—up to the frequency characterizing the molecular components—
 provides a mechanistic understanding of cell mechanics.\n\n1.	F. Rico\, L.
  Gonzalez\, I. Casuso\, M. Puig-Vidal\, S. Scheuring\, High-Speed Force Sp
 ectroscopy Unfolds Titin at the Velocity of Molecular Dynamics Simulations
 . Science 342\, 741 (2013).\n\n\n2.	A. Rigato\, A. Miyagi\, S. Scheuring\,
  F. Rico\, High-frequency microrheology reveals cytoskeleton dynamics in l
 iving cells. Nat Phys 13\, 771 (2017).\n
CATEGORIES:physbio,seminars
LOCATION:Amphi BLANDIN du LPS de la Faculté des Sciences d’Orsay (Bâtim
 ent 510)\, 15 Rue Georges Clemenceau\, orsay\, France
GEO:48.698187;2.181768
X-APPLE-STRUCTURED-LOCATION;VALUE=URI;X-ADDRESS=15 Rue Georges Clemenceau\,
  orsay\, France;X-APPLE-RADIUS=100;X-TITLE=Amphi BLANDIN du LPS de la Facu
 lté des Sciences d’Orsay (Bâtiment 510):geo:48.698187,2.181768
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