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TZID:Europe/Paris
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BEGIN:VEVENT
UID:1-121@lptms.universite-paris-saclay.fr
DTSTART:20130222T140000Z
DTEND:20130222T140000Z
DTSTAMP:20130131T144012Z
URL:http://www.lptms.universite-paris-saclay.fr/seminars/physics-biology-i
 nterface-seminar-jean-francois-allemand/
SUMMARY:Physics-Biology interface seminar : Jean-François Allemand - Moyen
  Amphi\, Building 510\, Université Paris-Saclay Orsay - 22 Fév 13 14:00
DESCRIPTION:In vitro &amp\; in vivo single molecule approaches to DNA repli
 cation \nJean-François Allemand (École normale supérieure)\nDNA replic
 ation is an essential step of the cell cycle. Despite many biochemical stu
 dies\, physical approaches can still bring new informations on some proces
 s of DNA replication.In vitro\, using micromanipulation tools\, we have sh
 own that force can trigger some DNA polymerases into an exonuclease mode w
 here\, instead of synthesizing DNA\, the polymerase goes backward and remo
 ves nucleotides. This can lead to a mechanical method for DNA sequencing.I
 n vivo\, in E. coli\, using single molecule fluorescence techniques we hav
 e quantified the real time composition of the replisome\, the machinary th
 at copies DNA. We observed a time variation of the composition of the repl
 isome with a number of DNA polymerases that varies from 2 to 3. We will se
 e how these fluctuations are related to the synthesis of the so called Oka
 zaki fragments. We will describe what happens to this scenario when the re
 plication is blocked under specific conditions. 
LOCATION:Moyen Amphi\, Building 510\, Université Paris-Saclay Orsay\, 15 R
 ue Georges Clemenceau\, orsay\, France
GEO:48.698187;2.181768
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  orsay\, France;X-APPLE-RADIUS=100;X-TITLE=Moyen Amphi\, Building 510\, Un
 iversité Paris-Saclay Orsay:geo:48.698187,2.181768
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