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TZID:Europe/Paris
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UID:0-787@lptms.universite-paris-saclay.fr
DTSTART:20211027T110000Z
DTEND:20211027T120000Z
DTSTAMP:20211004T092208Z
URL:http://www.lptms.universite-paris-saclay.fr/seminars/physics-biology-i
 nterface-seminar-renaud-poincloux/
SUMMARY:Physics-Biology interface seminar: Renaud Poincloux - Salle des sé
 minaires du FAST et du LPTMS\, bâtiment Pascal n°530 - 27 Oct 21 11:00
DESCRIPTION:Elasticity of dense actin networks produces nanonewton protrusi
 ve forces at macrophage podosomes\nRenaud Poincloux (IPBS\, Toulouse)\nAct
 in filaments assemble into force-generating systems that play pivotal role
 s in diverse cellular functions\, including cell motility\, adhesion\, con
 tractility and division. Thermodynamics and in vitro experiments showed th
 at the polymerization of single actin filaments generates forces in the 1-
 10 pN range. How networks of crosslinked actin filaments\, individually ge
 nerating piconewton forces\, are able to produce forces reaching tens of n
 anonewtons remains unclear. We used in situ cryo-electron tomography to un
 veil how the nanoscale architecture of macrophage podosomes enables basal 
 membrane protrusion. We show that the sum of the actin polymerization forc
 es at the membrane is not sufficient to explain the protrusive forces gene
 rated by podosomes. Quantitative analysis of podosome organization demonst
 rates that the core is composed of a dense network of bent actin filaments
  storing elastic energy. Theoretical modelling of the network as a spring-
 loaded elastic material reveals that it exerts forces of up to tens of nan
 onewtons\, similar to those evaluated experimentally. Thus\, taking into a
 ccount not only the interface with the membrane but also the bulk of the n
 etwork is crucial to understand force generation by actin machineries. Our
  integrative approach sheds light on the elastic behavior of dense actin n
 etworks and opens new avenues to understand force production inside cells.
CATEGORIES:physbio,seminars
LOCATION:Salle des séminaires du FAST et du LPTMS\, bâtiment Pascal n°53
 0\, rue André Riviere\, Orsay\, 91405\, France
X-APPLE-STRUCTURED-LOCATION;VALUE=URI;X-ADDRESS=rue André Riviere\, Orsay\
 , 91405\, France;X-APPLE-RADIUS=100;X-TITLE=Salle des séminaires du FAST 
 et du LPTMS\, bâtiment Pascal n°530:geo:0,0
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