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TZID:Europe/Paris
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BEGIN:VEVENT
UID:0-721@lptms.universite-paris-saclay.fr
DTSTART:20200129T110000Z
DTEND:20200129T120000Z
DTSTAMP:20200106T154445Z
URL:http://www.lptms.universite-paris-saclay.fr/seminars/physics-biology-i
 nterface-seminar-stephane-vassilopoulos-2/
SUMMARY:Physics-Biology interface seminar: Stéphane Vassilopoulos - Salle 
 des séminaires du FAST et du LPTMS\, bâtiment Pascal n°530 - 29 Jan 20 
 11:00
DESCRIPTION:Native membrane visualization by meta-replica electron microsco
 py\nStéphane Vassilopoulos (Sorbonne Université)\nIn cell biology\, part
 icular Importance is given to developing new methods of sample preparation
  that will achieve a more natural appearance of samples in the microscope.
  To accomplish this\, scientists have developed the metal-replica electron
  microscopy (EM) technique directly on unroofed cultured cells. This parti
 cular EM technique permits visualization of the inner side of cell membran
 es and their components with an extremely high resolution using transmissi
 on EM. Metal-replicas have profoundly shaped our understanding of the func
 tional morphology of the cell and used to capture several different cellul
 ar processes including remodeling of cytoskeletons\, membrane trafficking\
 , cell-to-cell contacts and formation of extracellular matrices. During th
 is presentation\, I will outline the history of this particular EM techniq
 ue and describe different methodologies and key applications in cell biolo
 gy with a particular emphasis on visualization of actin filament networks 
 at the cell cortex.\n\nSelected publications:\n\nVassilopoulos S*\, Gibaud
  S\, Jimenez A\, Caillol G\, Leterrier C. Ultrastructure of the axonal per
 iodic scaffold reveals a braid-like organization of actin rings. Nat Commu
 n. 2019 Dec 20\;10(1):5803.\nFerrari R*\, Martin G\, Tagit O\, Guichard A\
 , Cambi A\, Voituriez R\, Vassilopoulos S*\, Chavrier P*. MT1-MMP directs 
 force-producing proteolytic contacts that drive tumor cell invasion. Nat C
 ommun. 2019 Oct 25\;10(1):4886.\n\nFranck A\, Lainé J\, Moulay G\, Lemerl
 e E\, Trichet M\, Gentil C\, Benkhelifa-Ziyyat S\, Lacène E\, Bui MT\, Br
 ochier G\, Guicheney P\, Romero N\, Bitoun M\, Vassilopoulos S*. Clathrin 
 plaques and associated actin anchor intermediate filaments in skeletal mus
 cle. Mol Biol Cell. 2019 Mar 1\;30(5):579-590.\nRandrianarison-Huetz V\, P
 apaefthymiou A\, Herledan G\, Noviello C\, Faradova U\, Collard L\, Pincin
 i A\, Schol E\, Decaux JF\, Maire P\, Vassilopoulos S\, Sotiropoulos A. Sr
 f controls satellite cell fusion through the maintenance of actin architec
 ture. J Cell Biol. 2018 Feb 5\;217(2):685-700.\n\nElkhatib N\, Bresteau E\
 , Baschieri F\, Rioja AL\, van Niel G\, Vassilopoulos S\, Montagnac G. Tub
 ular clathrin/AP-2 lattices pinch collagen fibers to support 3D cell migra
 tion. Science. 2017 Jun 16\;356(6343).\n\nVassilopoulos S*\, Gentil C\, La
 iné J\, Buclez PO\, Franck A\, Ferry A\, Précigout G\, Roth R\, Heuser J
 E\, Brodsky FM\, Garcia L\, Bonne G\, Voit T\, Piétri-Rouxel F\, Bitoun M
 . Actin scaffolding by clathrin heavy chain is required for skeletal muscl
 e sarcomere organization. J Cell Biol. 2014 May 12\;205(3):377-93.
CATEGORIES:physbio,seminars
LOCATION:Salle des séminaires du FAST et du LPTMS\, bâtiment Pascal n°53
 0\, rue André Riviere\, Orsay\, 91405\, France
X-APPLE-STRUCTURED-LOCATION;VALUE=URI;X-ADDRESS=rue André Riviere\, Orsay\
 , 91405\, France;X-APPLE-RADIUS=100;X-TITLE=Salle des séminaires du FAST 
 et du LPTMS\, bâtiment Pascal n°530:geo:0,0
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