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UID:0-639@lptms.universite-paris-saclay.fr
DTSTART:20181107T110000Z
DTEND:20181107T120000Z
DTSTAMP:20181025T174853Z
URL:http://www.lptms.universite-paris-saclay.fr/seminars/physics-biology-i
 nterface-seminar-willy-supatto/
SUMMARY:Physics-Biology interface seminar: Willy Supatto - Amphi BLANDIN du
  LPS de la Faculté des Sciences d’Orsay (Bâtiment 510) - 7 Nov 18 11:0
 0
DESCRIPTION:Live imaging of motile cilia to investigate left-right symmetry
  breaking in zebrafish embryos\nWilly Supatto (LOB\, École polytechnique)
 \nIn vertebrate embryos\, cilia-driven fluid flows are guiding left-right 
 body symmetry breaking within the left-right organizer (LRO). To investiga
 te the generation and sensing of flows\, it is required to quantify cilia 
 biophysical features in 3D and in vivo [1]. In the zebrafish embryo\, the 
 LRO is called the Kupffer’s vesicle (KV) and is a spheroid shape cavity\
 , which is covered with motile cilia distributed at its surface and orient
 ed in all directions of space. This transient structure varies in size and
  shape during development and from one embryo to the other. As a consequen
 ce\, the experimental investigation of cilia properties is challenging. It
  requires quantifying cilia features in vivo and in 3D and combining the d
 ata from different embryos to compare one embryo to the other and perform 
 statistical analyses.To reach this goal\, we devised an experimental workf
 low combining live 3D imaging using multiphoton microscopy\, image process
 ing\, and data registration to quantify cilia biophysical features\, such 
 as cilia density\, motility\, 3D orientation\, or length. We integrated su
 ch experimental features obtained in vivo into a fluid dynamics model and 
 a multiscale physical study of flow generation and detection. This strateg
 y enabled us to demonstrate how cilia orientation pattern generates the as
 ymmetric flow within the KV [2]. In addition\, we could investigate the ph
 ysical limits of flow detection to clarify which mechanisms could be relia
 bly used for body axis symmetry breaking [2]. Finally\, we discovered the 
 distribution of cilia orientation is asymmetric within the KV [3]. Importa
 ntly\, these results suggested that the asymmetric force detection could r
 esult from the cilium being sensitive to its own motion. Together\, this w
 ork sheds light on the complexity of left-right symmetry breaking and chir
 ality genesis in developing tissues.\n[1] From cilia hydrodynamics to zebr
 afish embryonic development. Supatto &amp\; Vermot\, Current Topics in Dev
 elopmental Biology 2011\n[2] Physical limits of flow sensing in the left-r
 ight organizer. Ferreira et al\, eLife 2017\n[3] Chiral cilia orientation 
 in the left-right organizer. Ferreira et al\, Cell Reports\, in press
CATEGORIES:physbio,seminars
LOCATION:Amphi BLANDIN du LPS de la Faculté des Sciences d’Orsay (Bâtim
 ent 510)\, 15 Rue Georges Clemenceau\, orsay\, France
GEO:48.698187;2.181768
X-APPLE-STRUCTURED-LOCATION;VALUE=URI;X-ADDRESS=15 Rue Georges Clemenceau\,
  orsay\, France;X-APPLE-RADIUS=100;X-TITLE=Amphi BLANDIN du LPS de la Facu
 lté des Sciences d’Orsay (Bâtiment 510):geo:48.698187,2.181768
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