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TZID:Europe/Paris
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BEGIN:VEVENT
UID:1-187@lptms.universite-paris-saclay.fr
DTSTART:20131220T140000Z
DTEND:20131220T150000Z
DTSTAMP:20131210T124637Z
URL:http://www.lptms.universite-paris-saclay.fr/seminars/physics-biology-i
 nterface-seminar-yang-si/
SUMMARY:Physics-Biology interface seminar: Yang Si - Amphi BLANDIN du LPS d
 e la Faculté des Sciences d’Orsay (Bâtiment 510) - 20 Déc 13 14:00
DESCRIPTION:Fluorescent Nano-objects For Bioimaging Applications\nYang Si (
 ENS Cachan)\nSpecial seminar: poster prize from the NOMBA workshop\n\nBact
 eria are the most abundant organisms in the world. Studying models of bact
 erial chromosome dynamics in the cytoplasm is very important to understand
  how bacteria adapt to different growth environments and in response to st
 imuli.  \n\nOptical labeling is one of the most common methodologies used 
 for bioanalytical purposes. The fundamental issues for any fluorescent mat
 erial are the same: brightness and stability. In the quest for very bright
  and stable labels\, novel polymer-based\, self-stabilized\, fluorescent n
 anoparticles (FNPs) and fluorescent polymer chains (FPCs) have been develo
 ped in the PPSM laboratory. They are brighter\, more stable\, photobleach 
 slowly and are more easily functionalized compared with other fluorescent 
 labels like GFP and QDs.  \nA methodology to insert these FNPs (60nm) into
  E.coli bacteria was developed. To control if the FNP are indeed internali
 zed\, we developed a protocol based upon FNP luminescence quenching by met
 hylene blue.\n\nBiotin conjugated FNPs could be used to study specific mem
 brane proteins. By using a strepdavidin-biotin link\, we made a “Sandwic
 h” to build a bridge between particles\, specific antibodies and bacteri
 a.\n\nNegatively charged FPCs can easily enter into E.coli bacteria. It is
  found that FPCs can label the cytoplasm but not the DNA\, which appears t
 o be more compact.  These unique properties will allow the study of DNA an
 d cytoplasm viscosity changes during bacterial growth.\n
LOCATION:Amphi BLANDIN du LPS de la Faculté des Sciences d’Orsay (Bâtim
 ent 510)\, 15 Rue Georges Clemenceau\, orsay\, France
GEO:48.698187;2.181768
X-APPLE-STRUCTURED-LOCATION;VALUE=URI;X-ADDRESS=15 Rue Georges Clemenceau\,
  orsay\, France;X-APPLE-RADIUS=100;X-TITLE=Amphi BLANDIN du LPS de la Facu
 lté des Sciences d’Orsay (Bâtiment 510):geo:48.698187,2.181768
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