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TZID:Europe/Paris
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BEGIN:VEVENT
UID:0-798@lptms.universite-paris-saclay.fr
DTSTART:20211110T110000Z
DTEND:20211110T120000Z
DTSTAMP:20211014T164329Z
URL:http://www.lptms.universite-paris-saclay.fr/seminars/physics-biology-i
 nterface-seminar-yves-gaudin/
SUMMARY:Physics-Biology interface seminar: Yves Gaudin - Salle des séminai
 res du FAST et du LPTMS\, bâtiment Pascal n°530 - 10 Nov 21 11:00
DESCRIPTION:Interactions between viral factories and cellular innate immuni
 ty\, a story of liquid biocondensates\nYves Gaudin (I2BC)\nReplication of 
 Mononegavirales (MNV) occurs in viral factories which form inclusions in t
 he host-cell cytoplasm. For rabies virus (RABV)\, those inclusions are cal
 led Negri Bodies (NBs). NBs have characteristics similar to those of liqui
 d organelles: they are spherical\, they fuse to form larger structures\, a
 nd they disappear upon hypotonic shock. Their liquid phase was confirmed b
 y FRAP experiments. Live-cell imaging indicates that viral nucleocapsids a
 re ejected from NBs and transported along microtubules to form either new 
 virions or secondary viral factories. \nWe developed several minimal syste
 ms (both cellular and acellular) allowing the formation of biomolecular co
 ndensates recapitulating NBs properties. Those minimal systems established
  RABV phosphoprotein (P) as the main regulator of the liquid liquid phase 
 separation (LLPS) and identified structural elements of RABV nucleoprotein
  and P that are key in this process. Formation of liquid viral factories b
 y LLPS has been extended to other MNV. This is a paradigm change in the fi
 eld of MNV replication that invites us to revisit the interplay between vi
 ral factories and innate cellular immunity.\nAs an example\, we previously
  demonstrated that stress granules\, which are also liquid biomolecular co
 ndensates containing microbes-associated-molecular-patterns recognition re
 ceptors acting as sensors of RNA virus replication\, come into close conta
 ct with NBs\, exchange material with them\, but do not fully mix their con
 tent. We have now extended those observations to other components of the c
 ellular pathway leading to interferon production demonstrating a key role 
 of viral and cellular biomolecular condensates in innate immunity.   \n
CATEGORIES:physbio,seminars
LOCATION:Salle des séminaires du FAST et du LPTMS\, bâtiment Pascal n°53
 0\, rue André Riviere\, Orsay\, 91405\, France
X-APPLE-STRUCTURED-LOCATION;VALUE=URI;X-ADDRESS=rue André Riviere\, Orsay\
 , 91405\, France;X-APPLE-RADIUS=100;X-TITLE=Salle des séminaires du FAST 
 et du LPTMS\, bâtiment Pascal n°530:geo:0,0
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