Molecule motion inside secretory granules before and during exocytosis
Daniel Axelrod (University of Michigan)
SPECIAL DATE & TIME
Despite ~10% of the human genome being comprised of secretory proteins, little is known about the dynamics of proteins inside the secretory granule before and during fusion with the plasma membrane. This talk presents early results on measuring the diffusion coefficient of two proteins within that submicroscopic and closed space, and relates the results to secretion and local membrane deformation rates during the fusion event. The techniques used, TIR-FRAP, TIR-FCS, and pol-TIRF have general applicability, and the theory and practice of especially the first two is discussed.