Looking at transcription mechanisms with single molecule FRET
Emmanuel Margeat (Centre de Biochimie Structurale, Montpellier)
Exceptional seminar hosted by Karen Perronet
SPECIAL DATE, TIME AND LOCATION
Förster Resonance Energy Transfer (FRET) allows measuring the distance between two spectrally distinct fluorophores, in the 20-100 Å range. When monitored at the single molecule level, smFRET is useful in resolving subpopulations, or observing conformational changes as a function of time within single macromolecular complexes in vitro. I will describe here the methodologies used to measure FRET accurately on single biomolecular complexes, freely diffusing in solution or immobilized on surfaces. I will then focus on our studies on the mechanism of prokaryotic transcription such as antitermination control by the antiterminator LicT, and Rho-induced transcription termination.